The star headlined the Lesbian and Gay Pride Festival in Long Beach last month, which many took as a signal that she was coming out as a lesbian, following much speculation surrounding her sexuality.
Latifah has since dismissed the claim, insisting her appearance was merely to show her support of the LGBT community.
She told Entertainment Weekly, "That definitely wasn't the case. I've never dealt with the question of my personal life in public. It's just not gonna happen."
And Latifah reveals showing her support to the gay community is nothing new: "To me, doing a gay pride show is one of the most fun things. My first show that paid more than $10,000 was in a gay club on New Year's Eve in San Francisco. Tupac (Shakur) happened to be in town, so he came to kick it with me.
"This was the early '90s. And the boys were like, 'Take your shirt off, Tupac!' He wasn't doing that. But we had a blast in there."